|
KMID : 0380220070400030358
|
|
Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 3 p.358 ~ p.367
|
|
|
A Novel Mannose-binding Tuber Lectin from Typhonium divaricatum (L.) Decne (family Araceae) with Antiviral Activity Against HSV-II and Anti-proliferative Effect on Human Cancer Cell Lines
|
|
Luo Yongting
Xu Xiaochao
Liu Jiwei
Li Jian
Sun Yisheng
Liu Zhen
Liu Jinzhi
Damme Els Van
Balzarini Jan
Bao Jinku
|
|
|
Abstract
|
|
|
|
A novel mannose-binding tuber lectin with in vitro antiproliferative activity towards human cancer cell lines and antiviral activity against HSV-II was isolated from fresh tubers of a traditional Chinese medicinal herb, Typhonium divaricatum (L.) Decne by a combined procedure involving extraction, ammonium sulfate precipitation, ion exchange chromatography on DEAE-SEPHAROSE, CM-SEPHAROSE and gel-filtration on sephacryl S-200. The apparent molecular mass of the purified Typhonium divaricatum lectin (TDL) was 48 kDa. TDL exhibits hemagglutinating activity toward rabbit erythrocytes at 0.95 &mgr;g/ml, and its activity could be strongly inhibited by mannan, ovomucoid, asialofetuin and thyroglobulin. TDL showed antiproliferative activity towards some well established human cancer cell lines, e.g. Pro-01 (56.7 ± 6.8), Bre-04 (41.5 ± 4.8), and Lu-04 (11.4 ± 0.3). The anti-HSV-II activity of TDL was elucidated by testing its HSV-II infection inhibitory activity in Vero cells with TC50 and EC50 of 5.176 mg/ml and 3.054 &mgr;g/ml respectively. The full-length cDNA sequence of TDL was 1145 bp and contained an 813-bp open reading frame (ORF) encoding a 271 amino acid precursor of 29-kDa. Homology analysis showed that TDL had high homology with many other mannose-binding lectins. Secondary and three-dimensional structures analyses showed that TDL is heterotetramer and similar with lectins from mannose-binding lectin superfamily, especially those from family Araceae.
|
|
|
KEYWORD
|
|
|
Antiviral activity, Anti-proliferative activity, Molecular cloning, Purification and characterization, Sequence analysis, Typhonium divaricatum (L.) Decne lectin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|